IN-FRAME AMBER STOP CODON REPLACEMENT MUTAGENESIS FOR THE DIRECTED EVOLUTION OF PROTEINS CONTAINING NON-CANONICAL AMINO ACIDS: IDENTIFICATION OF RESIDUES OPEN TO BIO-ORTHOGONAL MODIFICATION.

In-frame amber stop codon replacement mutagenesis for the directed evolution of proteins containing non-canonical amino acids: identification of residues open to bio-orthogonal modification.

Expanded genetic code approaches are a powerful means to add new and useful chemistry to proteins at defined residues positions.One such use is the introduction of non-biological reactive chemical handles for site-specific biocompatible orthogonal conjugation of proteins.Due to our currently limited information on the impact of non-canonical amino

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Auditory Discrimination in Autism Spectrum Disorder

Autism spectrum disorder (ASD) is increasingly common with 1 in 59 children in the United States currently meeting the diagnostic criteria.Altered sensory processing is typical in ASD, with auditory sensitivities being especially common; in particular, people with ASD frequently show heightened sensitivity to environmental sounds and a poor ability

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Cross-Scale Value Trade-Offs in Managing Social-Ecological Systems: The Politics of Scale in Ruaha National Park, Tanzania

Management of social-ecological systems takes place amidst complex governance processes and cross-scale institutional arrangements that are mediated through politics of scale.Each management scenario generates distinct cross-scale trade-offs in the distribution of pluralistic values.This study explores the hypothesis that conservation-oriented mana

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Valuable Public Speaking Skills for Legal Professionals

In the constant endeavour to improve their Knitwear public speaking skills, legal professionals must be aware of the main aspects that they have to control and of the framework that they may use for that purpose.The elements of oral communication, politeness strategies, persuasion strategies, the main coordinates of verbal and nonverbal communicati

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An Investigation into the Factors Influencing the Reduction of Construction Materials Wastage in Erbil City- KRG-Iraq

The aim of this study is to identify the factors affecting the construction material waste generation Chaise Lounge and to determine the magnitude of construction waste material in Erbil city.To achieve this study, a form of a questionnaire was prepared comprised of the factors influencing the waste generation of construction material, and assessme

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